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PULSin
PULSin:
an exciting new protein and antibody delivery reagent
Protein delivery is emerging as a technique that complements gene transfection and RNA interference for studying protein function and cellular pathways. PULSin is a new reagent for efficient delivery of functional proteins/antibodies to living cells. - Effective and robust delivery into the cytoplasm
- Delivery to a wide variety of cells including primary cells
- Highly efficient transfer
- Easy to use
Fig 1:R-phycoerythrin (1ug) was complexed with 4 ul of PULSin for 15 mins and added to NIH-3T3 cells in a 24-well plate. Live cells were observed by fluorescence microscopy after 16 hours. Cells successfully transfected with PULSin
Cell Lines |
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A549 |
CV-1 |
MLE 15
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BHK-21 |
HEK-293 |
NIH-3T3
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CaSki |
HeLa |
RAW 264.7
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CHO |
MCF-7 |
SiHa
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Jurkat |
K562 |
HEK-293
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Human keratinocytes |
HepaRG |
MEF
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Human visceral pre-adipocytes |
HepG2 |
3T3-L1
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Human hepatocytes |
THP-1 |
HeLa
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Human fibroblasts |
HUVEC |
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What is PULSin?
PULSin contains a proprietary cationic amphophile which forms non-covalent complexes with many proteins including antibodies. Complexes are internalised via anionic cell-adhesion receptors present on all cells and are released into the cytoplasm where they disassemble. The process is harmless to cells and delivers unmodified functional proteins. Fig2. Left: Formalin fixed HeLa cells stained with a FITC anti-Giantin (Linstedt and Hauri 1993). Right: Live HeLa cells 20 hours after incubation with the same antibody complexed to PULSin. |
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What sort of research will PULSin be useful for?
- Visualization of cellular components
- Inhibition of protein activity
- Protein delivery
- Study of cell signaling pathways
- Transcription factor-mediated gene regulation
Is the protocol complicated?
No- it's easy and fast!
- PULSin is ready-to-use and includes dilution buffer and a fluorescent positive control protein.
- The protocol is rapid: simply add protein to the reagents, incubate and add to cells.
- Cells can be analysed 4 hours post-delivery
Fig3. FACS analysis of HeLa cells after delivery of FITC-anti-alpha-tubulin antibody with PULSin and competitive reagents B and C.
What does the kit contain?
Contents
- PULSin reagent
- 20 µg of R-phycoerythrin protein (positive control)
- 20 ml or 4 x 20 ml of Hepes Buffer for protein dilution
Pricing for PULSin
Cat No |
Size |
Reactions |
Price £ |
Special offer until 31st July
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501-01 |
0.1ml |
6 reactions in a 6-well plate |
70 |
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501-04 |
0.4ml |
24 reactions in a 6-well plate |
234 |
187
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501-16 |
4 x 0.4ml |
96 reactions in a 6-well plate |
749 |
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Product details |
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Add to basket |
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Datasheet PDF |
References:
Kapatz, I, Remy J, Behr, J(2004) A model for non-viral gene delivery: through syndecan adhesion molecules and powered by actin. J.Gene. Med 6 (&):769-776 Cassinelli G et al (2006) Inhibition of c-Met and prevention of spontaneous metastatic spreading by the 2-indolinone RPI-1. Mol Cancer Ther 5 (():2388-97 Leshchyns'ka, I et al (2006) The adhesion molecule CHL1 regulates uncoating of clathrin-coated synaptic vesicles. Neuron 52(6):1011-1025 |
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