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May 17, 2008 ©Autogen Bioclear UK Ltd
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SpinClean Plasmid miniprep kits 

SpinClean Plasmid miniprep kits

Introduction
SpinClean™ Plasmid Miniprep Kit contains chaotropic salt that enhances the binding of DNA onto a glass surface and reagents for alkaline lysis for easy purification of plasmid DNA from bacteria. The column is packed with glass fiber and has enough surface area to bind up to 20ug of plasmid DNA. Purified plasmid DNA is ready for restriction enzyme digestion, ligation, transformation, sequencing reactions, and library screening

Features

  • Silica-based membrane bound spin column
  • Rapid and easy protocol
  • Phenol extraction and ethanol precipitation are not required.
  • High quality (OD260/280>1.8)
SSequencing Grade
  • Ready to use (added RNase and ethanol)
  • Use restriction enzyme analysis, automated fluorescent sequencing analysis, etc.
 
SpinClean™ Plasmid Miniprep Kits
Cat No 11050 (50)
Cat No 11200 (200)
 
Miniprep Spin Column
50 ea
200 ea
Collection Tube
50 ea
200 ea
Cell resuspension Solution (Sol. I)
13ml
50ml
Cell Lysis Solution (Sol. II)
13ml
50ml
Neutralisation Solution (Sol. III)
18ml
70ml
Denaturation Solution
25ml
100ml
Column Washing Solution (Sol. IV)
10ml
20ml
Elution Buffer
-
20ml
RNase A solution
50ul
50ul
Protocol
1
1
Handbook
1
1
Price
£42.00
£159.00
 Product details    Add to basket    Datasheet PDF  
Figure 1
Agarose gel analysis of plasmid DNA purified by SpinClean™ Plasmid Miniprep Kit M: Size Marker, A: company A, B: company B, C: company C, and M-biotech: Mbiotech 
Figure 2
Electropherogram revealing >700 bases of sequence from pGEM-T vector using Mbiotech Plasmid Miniprep Kit