jetPRIME, the NEW transfection reagent from Polyplus Transfection is highly efficient yet extremely gentle to cells.

Advantages
► LOW cytotoxicity
► Excellent cell viability
► Compatible with serum AND antibiotics
► High DNA transfection efficiency
► Excellent gene silencing
► Economical and simple to use

Convenient protocol
jetPRIME™ is an easy-to-use transfection reagent:
• Fast and easy to scale up and down (Fig. 1)
• Simple: 1 : 2 DNA to jetPRIME™ ratio (w/v)
• No medium changes required, hence time saving
• Compatible with serum and antibiotics 

Fig. 2 Transfection efficiency assessed by flowcytometry analysis in various cell lines following transfection of pCMV-GFP plasmid. Transfection was performed using 250 ng DNA and 0.5 µl jetPRIME™ per well in 24-well plate following the manufacturer ‘s recommendations. 

Fig. 3 MTT assay determined 24 h after transfection to assess cell viability. Transfection of HeLa cells was performed according to the manufacturer’s recommendations for each reagent. 

jetPRIME™ leads to over 90% knockdown of endogenous gene expression in a variety of cell lines. For example, jetPRIME™-mediated transfection of HeLa cells with10 nM siRNA duplexes targeting endogenous lamin A/C in HeLa cells reduces lamin A/C gene expression to barely detectable level (Fig. left)

Endogenous lamin A/C silencing using jetPRIME™. HeLa cells were transfected with 10 nM of 21-mer lamin A/C siRNA. After 48 h, lamin A/C silencing was assessed by immunofluorescence microscopy using an antibody against lamin A/C.

jetPRIME is also well suited to DNA/siRNA co-transfection. 

Phase contrast microscopy of Hela cells 24 hours after transfection according to the manufacturers recommended protocols. Cells transfected using jetPRIME remain healthy while major cytotoxicity is shown with those using the competitor.