contact our friendly technical team: freephone 0800 6526774
View cart - empty   Home   Cell culture serum News archive Technical support   Links   Data sheets
May 17, 2008 ©Autogen Bioclear UK Ltd
part of the Source BioScience plc group
RNAi Off Target Effects 

RNAi Off Target Effects?

RNA interference (RNAi) is an important tool for studying gene function. siRNA (double stranded short interfering RNAs) are delivered to the cell as synthetic duplexes or hairpin RNAs (shRNAs) and incorporated through the RISC complex leading to cleavage and degradation of the target mRNA.

A growing concern that inducing RNAi using siRNA or shRNA can give unexpected non-specific effects at mRNA and protein level such as off target mRNA degradation and protein regulation, and induction of the interferon response (Jackson AL & Linsley PS (2004), TRENDS in Genetics Vol. 20 No.11, Persengiev S et al 2004 RNA 10:12). 

Are off-target effects a problem for you?
Do you know if they are?

The potential for immunostimulatory ‘side effects’ should be taken into consideration when using RNAi. Researchers have tried to avoid the problem by using the latest generation of transfection reagent (e.g. INTERFERin from PolyPlus Transfection) that give good silencing at sub-nanomolar siRNA concentrations. INTERFERin - click here to find out more

BUT did you know that you can measure these effects?

InvivoGen have introduced a set of primers designed to detect an IFN-response by real time quantitative PCR.

IFNr qRT-Primers

Detection of the IFN Response to siRNA or shRNA
The IFNr qRT primers have been designed to measure the mRNA expression of 5 genes induced by IFNalpha:-
  • IFN beta
  • OAS1
  • MX1
  • GIP2
  • IFIT1
  • and housekeeping GAPDH control
The primers allow one -step or two step qRT-PCR in combination with SYBR Green I dye 
Cat no.
Product
Quantity
Price
 
rts-hifnr
IFNr qRT-Primers
1 kit 10um each sufficient for 100 reactions in 96 well plate
£202
 Product details    Add to basket    Datasheet PDF  

INTERFERin siRNA transfection reagent - less is more!

For gene silencing at subnanomolar siRNA concentrations!
To optimise your siRNA concentration and reduce non-specific effects try INTERFERin. more information and special offer!
Top Data sheets How to order Our Manufacturers Privacy statement Email a colleague About us Contact us Site map Legal