ISF-1 is a chemically defined optimised medium that has been developed for serum-free growth of mouse hybridoma cell lines and the production of monoclonal antibodies in flasks, roller bottles and agitated suspension cultures. Datasheet

Cells can be adapted to ISF-1 Serum-free medium either by rapid conversion or weaning. While rapid conversion may be successful for some types of cells, a gradual conversion is more likely to yield the desired result.

Two protocols are available:

Protocol 1:
for use with anchorage-dependent cells or cells that ae loosely attached and do not require trypsinisation. It involves the gradual dilution of the serum-containg medium with the serum-free medium.

Protocol 2:
may be used with both anchorage-dependent and independent cell types and begins with serum-free medium supplemented with serum. A gradual reduction in serum concentration is performed at each subculture until serum-free growth is achieved. Protocols