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INTERFERin
siRNA Transfection
Less is more.....
with INTERFERin you'll get more valuable data using less siRNA. Q: Why use an siRNA transfection reagent with 1nM siRNA? A: Because scientists want to obtain results they can rely on- studies show that using low siRNA concentrations avoids the unwanted toxic and off-target effects observed when transfecting siRNA at higher concentrations (Persengiev S et al 2004 RNA 10:12).
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- Great silencing at subnanomolar siRNA concentrations
- Over 90% gene silencing in a wide variety of cells
- Compatible with serum and antibiotics
- Simple protocol
- Excellent cell viability
- Ready to use
- Suitable for batch and reverse transfections
- NEW high throughput screening protocol available!
Protocols for adherent and suspension cells, and HTS |
Over 90% gene silencing in a wide variety of cells
For adherent cell lines or primary cells, 1 nanomolar or less siRNA concentration is sufficient to give more than 90% gene silencing, regardless of the supressed gene. for difficult to transfect suspension cell lines, 80% silencing can still be reached using 5nM siRNA. For both standard and HTS screening protocols see the product information below, or contact us for further information. Silencing Efficiency in selected cells using INTERFERin
Adherent cell lines (at 1 nM siRNA concentration) |
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A549 |
Luciferase |
>90%
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HeLa |
GAPDH / Lamin A/C |
>90%
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CaSki |
GAPDH / Lamin A/C |
>90%
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MCF7 |
GAPDH / Lamin A/C |
>90%
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NIH-3T3 |
Vimentin |
>90%
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SiHa |
GAPDH / Lamin A/C |
>90%
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Primary cells (at 1 nM siRNA concentration) |
GAPDH / Lamin A/C |
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Primary human fibroblasts |
GAPDH |
>90%
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Primary human hepatocytes |
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>90%
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Suspension cell lines (at 5 nM siRNA concentration) |
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K562 |
GAPDH |
>80%
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THP-1 |
GAPDH |
>80%
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Endogenous Lamin A/C silencing using INTERFERin
CaSki cells were transfected with 1nM of 21-mer siRNA duplexes matching the lamin A/C sequence using INTERFERin. After 48h, lamin A?C silencing efficiency was determined using immunofluoresence microscopy.
INTERFERin mediated siRNA transfection inhibits luciferase expression in A549-GL3Luc cells
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Cells were transfected in 24 well plates in the presence of serum with decreasing concentrations of Luciferase siRNA (GL3Luc) or control siRNA (mismatch GL2luc) duplexes using INTERFERin. Luciferase expression was measured after 48h. |
FREE samples!
Interested in testing INTERFERin?
Request your free sample today, please click on the link below and complete the form. Sample request form
Cat No |
Qty |
No of Transfections (per 24 well plate) |
Price �
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409-01 |
0.1ml |
50-100 |
29
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409-05 |
0.5ml |
250-500 |
115
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409-10 |
1ml |
500-1000 |
192
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409-50 |
5 x 1ml |
2500-5000 |
755
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Product details |
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Datasheet PDF |
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