Description
PlasmoTest™ is a cell-based colorimetric assay that exploits the ability of Toll-like receptor 2 to recognize mycoplasmas and to induce a signaling cascade leading to the activation of NF-kappaB and other transcription factors. In the presence of mycoplasmas, TLR2 expressed on the surface of HEK-Blue™-2 cells activates these transcription factors which in turn induce the secretion of sAP (secreted alkaline phosphatase), a reporter protein easily detectable by the purple/blue coloration of the HEK-Blue™ Detection medium.

• Simple - Requires only basic cell culture knowledge. No need for specific lab equipment. Results are easily determined with the naked eye or quantified with a spectrophotomete

• Rapid - Hands-on time less than 1 hour. Gives results after overnight incubation.

• Versatile - Detects all Mycoplasma and Acheloplasma species known to infect cell cultures, as well as other cell culture contaminants such as bacteria

• Sensitive - Detects 5.102-5.105 cfu/ml mycoplasma. No false positive: a positive result indicates the presence of a cell culture contaminan

• Complete - Contains the HEK-Blue™-2 cells and all the reagents needed to perform the assay, including positive and negative controls. Up to 500 samples can be tested with the kit. To perform further assays, only the reagents need to be re-ordered.

 

Procedure 

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