PlasmoTest is a cell-based colorimetric assay that exploits the ability of Toll-like receptor 2 to recognize mycoplasmas and to induce a signaling cascade leading to the activation of NF-kappaB and other transcription factors. In the presence of mycoplasmas, TLR2 expressed on the surface of HEK-Blue-2 cells activates these transcription factors which in turn induce the secretion of sAP (secreted alkaline phosphatase), a reporter protein easily detectable by the purple/blue coloration of the HEK-Blue Detection medium.

• Simple: requires only basic cell culture knowledge. No need for specific lab equipment. Results are easily determined with the naked eye or quantified with a spectrophotometer

• Rapid: hands-on time less than 1 hour. Gives results after overnight incubation.

• Versatile: detects all Mycoplasma and Acheloplasma species known to infect cell cultures, as well as other cell culture contaminants such as bacteria

• Sensitive: detects 5.10^2-5.10^5 cfu/ml mycoplasma. No false positive: a positive result indicates the presence of a cell culture contaminants

• Complete: contains the HEK-Blue-2 cells and all the reagents needed to perform the assay, including positive and negative controls. Up to 500 samples can be tested with the kit. To perform further assays, only the reagents need to be re-ordered.

 

Procedure 

Contents

• HEK-Blue-2 cells (3-5 x 10^6 cells): cells are shipped on dry ice. Upon receipt, store HEK-Blue-2 cells at -80°C.
  
• HEK-Blue-2 Selection (4 x 2 ml) - Antibiotic mix
  
• Normocin (4 x 1 ml)
  
• HEK-Blue Detection (2 x 50 ml)
  
• HEK-Blue water (120 ml)
  
• Positive control - Not a living Mycoplasma
  
• Negative control

 

Mycoplasma may affect 63% of cell cultures; they cannot be detected by visual inspection and can alter cell physiology and gene expression.
Plasmocin is a new generation of bactericidal antibiotic preparation strongly active on mycoplasma- infected cells. More information on Plasmocin

Buy a PlasmoTest before 31st July 2009 and receive a FREE Plasmocin (cat no ant-mpt) for the treatment and elimination of mycoplasma in cell culture.

Please quote FREEMPT when placing your order