PhosphoCruz Protein Purification System, sc-24964

• Affinity purification system devised to enrich/isolate phosphor-proteins from cell lysates and tissue extracts for phosphorylation studies; proteins that carry a phosphate group are retained on the column while non-phosphorylated and undesired contaminants pass through
  
• Typical phospho-enriched protein yield is 5-10% of total protein
  
• Contains materials required for six individual phospho-protein enrichment procedures including six single-use columns, 40 ml 5X Binding/Wash Buffer, 30 ml Eluting Buffer, 200 µl each Phosphatase Inhibitor Cocktails A and B, and six ultra-filtration concentrators for concentrating phosphor-enriched protein post-purification
  
• Additional 5X Binding/Wash Buffer (sc-24965) and Ultra Filtration Concentrators (sc-24966) also available.

 

EGF treated A-431 cells were processed by using the PhosphoCruzTM Protein Purification System sc-24964. Fig. 1: Blots were probed with phosphotyrosine antibody, sc-508, detecting tyrosine phosphorylated EGFR. Lanes A/B: unbound column fractions. Lanes C/D: bound column (phosphoprotein enriched) fractions. Lanes E/F: whole cell lysates prepared from untreated and EGF treated cultures, respectively. Fig. 2: The same samples shown in Fig. 1 were analyzed with antibody to HSP 60, sc-13115. 

Price: £175 for 6 purifications